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Team:Warsaw/Calendar-Main/6 May 2008
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<p>Piotr: <br> | <p>Piotr: <br> | ||
| - | Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">electro-</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.<br> | + | 1. Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">electro-</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.<br> |
| - | Setup of overnight cultures (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>) on LB broth with proper antibiotics.<br> | + | 2. Setup of overnight cultures (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>) on LB broth with proper antibiotics.<br> |
Michał L.: <br> | Michał L.: <br> | ||
| - | <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> Rosetta) - template DNA for PCR to obtain T7 polymerase DNA.<br> | + | 1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> Rosetta) - template DNA for PCR to obtain T7 polymerase DNA.<br> |
</p> | </p> | ||
Revision as of 07:14, 29 September 2008
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