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Team:Warsaw/Calendar-Main/6 May 2008
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<h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a> construct</h3> | <h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a> construct</h3> | ||
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Preparation of pMPMT5+AID constructPiotr, Weronika
Isolation of genomic DNAMichał L.
Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from E. coli TOP10 strain was used as a negative control for PCR. Fig. 1. 
Fig. 1. Probes of isolated genomic DNA after mechanical destruction. 1 and 3-E.coli Rosetta DNA; 2-DNA ladder; 4-E.coli TOP10 DNA.
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