1. Preparation of electro- and chemocompetent bacteria E. coli TOP10 (lot of fun)
2. Isolation of genomic DNA (E.coli Rosetta)
3. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320)

Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since.
Digestion was conducted with BamHI