Team:Warsaw/Calendar-Main/6 May 2008

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Preparation of pMPMT5+AID construct

Piotr

  1. Preparation of electro- and chemocompetent bacteria E. coli TOP10.
  2. Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320) on LB broth with proper antibiotics.

Isolation of genomic DNA

Michał L.

Isolation of genomic DNA (E.coli Rosetta and TOP10) - template DNA for PCR to obtain T7 polymerase DNA. DNA isolated from E. coli TOP10 strain was used as a negative control for PCR. Fig. 1.

Fig. 1. Probes of isolated genomic DNA after mechanical destruction. 1 and 3-E.coli Rosetta DNA; 2-DNA ladder; 4-E.coli TOP10 DNA.

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