Team:Warsaw/Calendar-Main/6 October 2008
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- | <h3>Preparation of | + | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103009>linker_alpha (BBa_K103009)</a></h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinLSXNE">LinLSXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaPSpe">AlphaPSpe</a> | <ol><li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#LinLSXNE">LinLSXNE</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaPSpe">AlphaPSpe</a> | ||
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- | <h3>Preparation of | + | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103013>linker_omega (BBa_K103013)</a></h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p>Inoculation of some colonies which grown on plate - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103013>linker_omega (BBa_K103013)</a> to liquid LB + kanamycin.</p> | <p>Inoculation of some colonies which grown on plate - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> + <a href=http://partsregistry.org/Part:BBa_K103013>linker_omega (BBa_K103013)</a> to liquid LB + kanamycin.</p> | ||
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- | <h3>Preparation of | + | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103016>OmpA-linker-omega-linker (BBa_K103016)</a></h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<ol> | <ol> |
Revision as of 10:37, 27 October 2008
Preparation of linker_alpha (BBa_K103009)Michał K.
Preparation of linker_omega (BBa_K103013)Michał K.Inoculation of some colonies which grown on plate - pSB2K3 + linker_omega (BBa_K103013) to liquid LB + kanamycin. Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Preparation of vector for pT7 constructsMichał K.Overnight selfligation of pET15b+OmpA_omega without XbaI. Preparation of BioBricksMichał K.
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