Team:Warsaw/Calendar-Main/9 October 2008

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Preparation of BioBricks

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (RFP(?????)+ AID and pACYC177 + pET15b+OmpA_omega without XbaI).
  2. Control digest of isolated RFP(?????)+ AID plasmids with EcoRI and PstI (Orange buffer) and pET15b+OmpA_omega without XbaI plasmids with XbaI and BamHI (Tango buffer). Gel electrophoresis - proper clones found for both ligations.

Piotr

  1. Isolation of plasmid from culture inoculated on previous day (3kb??????) + pLac_OmpA_omega (without EcoRI site).
  2. Control digest of isolated (3kb??????) + pLac_OmpA_omega with EcoRI and PstI (Orange buffer) proper clones found.

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