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Team:Warsaw/Calendar-Main/9 September 2008
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<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega</h3><h4>Piotr</h4> | <h3>Purification of proteins: A-alpha, Z-alpha and Z-omega</h3><h4>Piotr</h4> | ||
| - | <p>Measurement of concentration of | + | <p>Measurement of concentration of all proteins (<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">BCA method</a>). </p> |
<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | <h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | ||
| - | <p>Inoculation all variants of OmpA proteins to LB with inductor (0 | + | <p>Inoculation of all variants of OmpA proteins to LB with inductor (0.25 mM IPTG) and kanamycin.</p> |
Revision as of 21:59, 15 October 2008
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Purification of proteins: A-alpha, Z-alpha and Z-omegaPiotrMeasurement of concentration of all proteins (BCA method). Tests for ampicillin resistance given by protein added to mediumPiotrInoculation of all variants of OmpA proteins to LB with inductor (0.25 mM IPTG) and kanamycin.
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