Team:Waterloo/Notebook/Oligos

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Description Oligo Name Sequence Length
BioBrick sequencing/colPCR primer (fwd) iGEMvf2 TGCCACCTGACGTCTAAGAA 20
BioBrick sequencing/colPCR primer (rev) iGEMvr ATTACCGCCTTTGAGTGAGC 20
Primer for Lac promoter (fwd) PLacF TTATGCTTCCGGCTCGTATG 20
Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2) iGEMpre+rbs TTTCTCCTCTTTCTCTAGATGCGG 24
Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr) PrefixRBS-F GGCCGCTTCTAGAGAAAGAGG 21
Gene 5 ORF from T7 phage (fwd; Prefix adapter) T7gene5-F CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA 46
Gene 5 ORF from T7 phage (rev; Suffix adapter) T7gene5-R CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG 51
Gene 6 ORF from T7 phage (fwd; Prefix adapter) T7gene6-F CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA 46
Gene 6 ORF from T7 phage (rev; Suffix adapter) T7gene6-R CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG 43
PmeIR ORF (fwd) PmeIR-F ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG 50
PmeIR ORF (rev) PmelR-R CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG 52
pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAF AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA 71
pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) recAR CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG 71
{B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR) RBST7g6-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC 61
T7gene6 ORF (rev) T7gs-R CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC 48
T7g6-RXO-RBS CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC 53
RBSpmeI-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 61
RBSpmeI-FXO TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG 48
pmeI-R CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG 45
R0011-F GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC 38
R0011RBS-RXO CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC 47
RBSC0051-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 59
RBSC0051-FXO TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC 46
C0051-R CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG 40
C0051-RXO GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG 36
B0015-FXO TAATAATACTAGAGCCAGGCATCAAATAAAACG 33
B0015-R CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC 39
RFP-RBS-F GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG 58
RFP-RXO GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG 34
Plac-RBS-F GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACATACTAGAGAAAGAGGAGAAATACTAG 103