Biobrick

List of Biobricks:

BBa_K094100, BBa_K094101, BBa_K094102, BBa_K094103, BBa_K094104, BBa_K094105, BBa_K094106, BBa_K094110, BBa_K094111, BBa_K094112, BBa_K094113, BBa_K094120, BBa_K094130, BBa_K094141, BBa_K094150, BBa_K094151

BBa_K094100
cheZ: This part contains the cheZ operon coding region without an rbs nor terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.

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BBa_K094101
plambda(R-O12)-rbs-cheZ: This part contains a lambda promoter that is constitutively on and can be repressed by CI protein. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.

BBa_K094102
pTetR-rbs-cheZ: This part uses BBa_J61003 as a backbone and cheZ as the inserted gene. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ operon lacks a terminator.

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BBa_K094103
plux-rbs-cheZ-terminator: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.

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BBa_K094104
pLacI/ara-1-rbs-mRFP-double terminator: This part is adapted from part BBa_J04450 by changing the promoter pLacI to promoter pLacI/ara-1. This part can test the leaky of promoter plac/ara-1.

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BBa_K094105
plambda(R-O12)-rbs-cheZ:-rbs-mRFP1-double terminator: This part can be used to measure the gene expression of cheZ under the control of promoter plambda(R-O12).

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BBa_K094106
Plux-rbs-CI-terminator-plambda-rbs-cheZ: This part encodes a CI protein that is used to repress lambda promoter. The part is controlled by the lux promoter that can be induced by luxR protein under high AHL concentration. In this part, the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances, the cheZ gene express constitutively.

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BBa_K094110
cheY*: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.

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BBa_K094111
pTetR-rbs-cheY*: This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part lacks a terminator.

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BBa_K094112
luxR-rbs-luxI-terminator: : The bacterial luciferin-luciferase system is encoded by a set of genes labelled the Lux operon. In V. fischeri five such genes (LuxCDABE) have been identified as active in the emission of visible light, and two genes (LuxR and LuxI) are involved in regulating the operon. This part can be utilized as a part of quorum sensing system.

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BBa_K094113
lacZ: This is a reporter gene that codes for beta galactosidase.

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BBa_K094120
pLacI/ara-1: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.

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BBa_K094130
rbs-mRFP1-terminator: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.

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BBa_K094141 <
pLacIq-rbs-LacIq-terminator: LacIQ can repress promoter lac. This part contains a mutated pLacIq promoter which enables high level of transcription. This ensures effective repression of pLacI promoter that might be present.

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BBa_K094150
pLacI/ara-1-rbs-luxI-luxR-terminator: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR protein as the AHL density sensor.

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BBa_K094151
pLacI/ara-1-rbs-luxI-terminator: The luxI operon is controlled by promoter LacI/ara-1.