Template:Team:UC Berkeley/Notebook/MJV construction

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''Construction of <Cre! Basic Part K112100''
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'''Construction of <Cre! Basic Part K112100'''
<pre>
<pre>
PCR mv001F/mvoo1R on pSB1A2-I13521    (1261bp, EcoRI/BamHI/DpnI)
PCR mv001F/mvoo1R on pSB1A2-I13521    (1261bp, EcoRI/BamHI/DpnI)

Revision as of 21:49, 4 June 2008

Construction of <Cre! Basic Part K112100

PCR mv001F/mvoo1R on pSB1A2-I13521     (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112800
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ca8373	Forward Biobricking of mRFP
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
ca8374	Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc