Template:Team:UC Berkeley/Notebook/MJV construction/corrected assembly vector

From 2008.igem.org

Removing attR2 point mutation in pBjh1601CA 

PCR mv042F/mv042R on pBjh1601CA      (3861bp, gp, BglII/MfeI)
Sub in pBca1256-attR2                 (EcoRI/BamHI/DpnI, 2472+151, S)
Product is pK112128CA
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mv042F	Forward oligo for pBjh1600CA to patch attR2 (BglII)
gcagtAGATCTcttacAGATCCcggttatcc
mv042R	Reverse oligo for Bjh1600CA to patch attR2 (MfeI)
gcagtCAATTGctgcagactggctgtg
Retrieved from "http://2008.igem.org/Template:Team:UC_Berkeley/Notebook/MJV_construction/corrected_assembly_vector"