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User:University of Washington/15 August 2008
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-got colonies of transformation of LuxR/GFP + promoter circuit. Next is to overnight(Sunday?) and sequence(Monday?) | -got colonies of transformation of LuxR/GFP + promoter circuit. Next is to overnight(Sunday?) and sequence(Monday?) | ||
| - | ==Yeast Shuttle Plasmid== | + | ==Yeast Shuttle Plasmid (Alec)== |
- Heat inactivated the restriction digest reactions for J63005 (vector) and J33202 (insert) at 80 degrees Celsius for 20 minutes. | - Heat inactivated the restriction digest reactions for J63005 (vector) and J33202 (insert) at 80 degrees Celsius for 20 minutes. | ||
Latest revision as of 23:23, 15 August 2008
MG1655Z1(Faifan)
-streaked culture from glycerol stock on LB plate and incubated at 42 degree Celsius(Ingrid's lab).
LuxR from AraC and TetR(Faifan)
-got colonies of transformation of LuxR/GFP + promoter circuit. Next is to overnight(Sunday?) and sequence(Monday?)
Yeast Shuttle Plasmid (Alec)
- Heat inactivated the restriction digest reactions for J63005 (vector) and J33202 (insert) at 80 degrees Celsius for 20 minutes.
- Ran a gel with the vector and insert fragments, and circular and linearized pAC88.
- Gel purified the vector and insert fragments using Qiagen Gel Purification kit.
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