User:University of Washington/20 August 2008

From 2008.igem.org

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-picked 4 colonies and streaked out on Amp, Cam, Kan, and Tet plates.
-picked 4 colonies and streaked out on Amp, Cam, Kan, and Tet plates.
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==LuxR from pLac==
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-Minipreps of parts R0010 and I0462 were digested for assembly and ran on a gel to separate fragments.
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-The R0010 vector and I0462 insert fragments were excised from the gel.
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Latest revision as of 21:57, 22 August 2008

Contents

LuxR from AraC and TetR(Faifan)

Elowitz

- ran gel for digested P1010(KAN), E0240, I0462, BBa_Elowitz

- cut out the bands and did Gel purification

MG1655Z1(Faifan)

-received culture on non-selective plate from Ingrid

-picked 4 colonies and streaked out on Amp, Cam, Kan, and Tet plates.

LuxR from pLac

-Minipreps of parts R0010 and I0462 were digested for assembly and ran on a gel to separate fragments.

-The R0010 vector and I0462 insert fragments were excised from the gel.



Back to Team:University_of_Washington/Notebook#Notebook