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User:University of Washington/24 July 2008
From 2008.igem.org
(Difference between revisions)
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- ran gel for R0080 (miniprepped yesterday) to see if AraC DNA in 6 tubes had same length. All except tube two appeared to be around 2 kb(as expected), so they were combined into one. | - ran gel for R0080 (miniprepped yesterday) to see if AraC DNA in 6 tubes had same length. All except tube two appeared to be around 2 kb(as expected), so they were combined into one. | ||
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| + | ==LuxR from pLac== | ||
| + | |||
| + | -Cells transformed with R0010 and E0420 digest successfully grew on amp plate. Overnight culture started for sequencing. | ||
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| + | -Overnight culture of DH5a+lacIq strain started. Needed for future transformations. | ||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 23:34, 24 July 2008
LuxR from AraC and TetR
- miniprepped mutated AraC (reaction1 x 4) (reaction2 x 4) in 30 ul EB.
- ordered sequencing for mutated AraC with forward primers.
- ran gel for R0080 (miniprepped yesterday) to see if AraC DNA in 6 tubes had same length. All except tube two appeared to be around 2 kb(as expected), so they were combined into one.
LuxR from pLac
-Cells transformed with R0010 and E0420 digest successfully grew on amp plate. Overnight culture started for sequencing.
-Overnight culture of DH5a+lacIq strain started. Needed for future transformations.
Back to Team:University_of_Washington/Notebook#Notebook
