User:University of Washington/24 July 2008

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LuxR from AraC and TetR

- miniprepped mutated AraC (reaction1 x 4) (reaction2 x 4) in 30 ul EB.

- ordered sequencing for mutated AraC with forward primers.

- ran gel for R0080 (miniprepped yesterday) to see if AraC DNA in 6 tubes had same length. All except tube two appeared to be around 2 kb(as expected), so they were combined into one.

LuxR from pLac

-Cells transformed with R0010 and E0420 digest successfully grew on amp plate. Overnight culture started for sequencing.

-Overnight culture of DH5a+lacIq strain started. Needed for future transformations.

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