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User:University of Washington/25 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: == Yeast Shuttle Plasmid == -Obtained plate of yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0) -Incubated yeast at 30 degrees Celsius overnight [...) |
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| + | == LuxR under AraC and TetR == | ||
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| + | |||
| + | -Obtained BBa_R0040 (TetR), BBa_R0080 (AraC) | ||
| + | |||
| + | -transformed and plated with Amp | ||
[[Team:University_of_Washington/Notebook#Notebook]] | [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 22:07, 26 June 2008
Yeast Shuttle Plasmid
-Obtained plate of yeast strain BY4741 (MATalpha, ΔBar1::Kan MX, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0)
-Incubated yeast at 30 degrees Celsius overnight
LuxR under AraC and TetR
-Obtained BBa_R0040 (TetR), BBa_R0080 (AraC)
-transformed and plated with Amp
Team:University_of_Washington/Notebook#Notebook
