User:University of Washington/7 August 2008

From 2008.igem.org

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= Yeast Shuttle Plasmid ==
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- Obtained yeast strain K. lactis
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- Obtained primers for amplification of LAC4-LAC12 region of K. lactis genome
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- PCR'd the K. lactis with primers
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- Made glyercol stocks of BBa_J63005 and BBa_J33202
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== BioBrick Promoter Measurements ==
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- Inoculated overnight cultures with colony isolates of TOP10 for promoter constructs I20260, I20259, I20261, I20267, and empty TOP10 cells.
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Revision as of 22:29, 7 August 2008

Yeast Shuttle Plasmid =

- Obtained yeast strain K. lactis

- Obtained primers for amplification of LAC4-LAC12 region of K. lactis genome

- PCR'd the K. lactis with primers

- Made glyercol stocks of BBa_J63005 and BBa_J33202

BioBrick Promoter Measurements

- Inoculated overnight cultures with colony isolates of TOP10 for promoter constructs I20260, I20259, I20261, I20267, and empty TOP10 cells.

Back to Team:University_of_Washington/Notebook#Notebook

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