User:University of Washington/7 August 2008

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Yeast Shuttle Plasmid

- Obtained yeast strain K. lactis

- Obtained primers for amplification of LAC4-LAC12 region of K. lactis genome

- PCR'd the K. lactis with primers

- Made glyercol stocks of BBa_J63005 and BBa_J33202

BioBrick Promoter Measurements

- Inoculated overnight cultures with colony isolates of TOP10 for promoter constructs I20260, I20259, I20261, I20267, and empty TOP10 cells.

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