Utah State/16 October 2008

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Isolated 4 cultures of I13600, E/P digested (40 ul reactions), ran out on gel, cut our from gel (4 x pSB1A3).

Performed PCR on 3 possible biobricks (#9, #4 and #7, #6 of 10 tubes of possible biobricks) from 10/11/2008 electrophoresis pictures & ran out on gel to confirm - looks good! Cut out from gel just in case they would be needed.

Reinoculated all 10 potential biobricks because they were left in the freezer.

Transformed pSB3K3 with ccdb toxin into special competent cells.