Utah State/17 July 2008

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Mini-scale preparation for DNA sequencing by CTAB-DNA precipitation was performed for all constructs except I20260 (the full GFP construct sent later by iGEM) up to the point of pellet removal. Procedures were done to this point to facilitate doing electrophoresis tomorrow for all constructs. The I20260 transformation worked very well and will be brought to the same point as the other 10 constructs tomorrow, after which preparation for DNA sequencing, enzymatic digestion, and electrophoresis will be completed for all the constructs. Also, the I13521 (RFP) streak plate produced the characteristic red-hued bacteria, thereby showing the correct preparation of the antibiotic agar plates.