Utah State/20 October 2008

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Six ligations were performed, three with new T4 ligase and three with older T4 ligase: -35 Pro in pSB1A3, 5'F/SD R in pSB1A3, and phaB in pSB1A3.

Cryostocks were made of 5'F/ATG R, 2x 5'F/TC R, and -35F/TC R