Utah State/22 October 2008

From 2008.igem.org

(Difference between revisions)
(New page: PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's in...)
 
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Transformations from yesterday produced bacterial lawns.  New ampicillin solution was made and used in new plates. 
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PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S.  This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's instructions.
PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S.  This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's instructions.

Latest revision as of 17:53, 24 October 2008

Transformations from yesterday produced bacterial lawns. New ampicillin solution was made and used in new plates.

PCR was performed for 5'F/SD R and -35F/ATG R and digested with E/S. This required a sequential digestion of Spe1 first then EcoR1 + additional 10X buffer O according to manufacturer's instructions.