Virginia/17 July 2008

From 2008.igem.org

(Difference between revisions)
(Goals)
(Goals)
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**this should allow us to do an SDS-PAGE on this first bioplastic gene
**this should allow us to do an SDS-PAGE on this first bioplastic gene
**we're not sure if a lack of terminator on the gene will be problematic
**we're not sure if a lack of terminator on the gene will be problematic
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*Assemble A6(R0010+A4) with a terminator(B0015) which we plan on also growing in a LAC liquid culture tomorrow
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*Digest and Assemble A6(R0010+A4) with a terminator(B0015) which we plan on also growing in a LAC liquid culture tomorrow
*Grow the measurement plasmid(psb1a10) on a plate with arabinose and in overnight liquid broth with and without arabinose
*Grow the measurement plasmid(psb1a10) on a plate with arabinose and in overnight liquid broth with and without arabinose
**plating with arabinose will prove that both genes in the vector will fluoresce
**plating with arabinose will prove that both genes in the vector will fluoresce

Revision as of 15:09, 17 July 2008

Overnight Results

  • only plate with R0010 +A4 grew which is the same result as from the night before
    • Which leads us to think our assemblies have some problem
  • the assembly of BP2 and BP3 with the RBS(B0034) has failed twice in a row we need to look at the gel and make sure we are not making any false assumptions about the genes we have

Goals

  • Grow E0240 in liquid broth from originally growing plate that came from the new DNA spot in the most recent newsletter
    • we need to prove that this gene will glow green before we try to make it fluoresce in an assembly
  • Use overnight broth of R0010 + A4 from night before to miniprep and grow in a LAC liquid culture
    • this should allow us to do an SDS-PAGE on this first bioplastic gene
    • we're not sure if a lack of terminator on the gene will be problematic
  • Digest and Assemble A6(R0010+A4) with a terminator(B0015) which we plan on also growing in a LAC liquid culture tomorrow
  • Grow the measurement plasmid(psb1a10) on a plate with arabinose and in overnight liquid broth with and without arabinose
    • plating with arabinose will prove that both genes in the vector will fluoresce
    • overnight broth will allow us to prepare DNA by miniprepping so that we can insert terminators in between fluorescence
    • Overnight broth with arabinose will be a double check to our work because we are not confident in our ability to make plates with arabinose
  • Try assembling and plating BP2 and BP3 with the medium strength RBS instead of standard
  • Start over with BP2 and BP3 grow a broth from the cells that were sent to us


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