Virginia/6 September 2008

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(New page: *Cut parts for A70-A72 **(RBS+BP) assemblies were cut E,S **B0015 was cut E,X **>Standard assembly *Also, used old cuts of A58(E,S) and A59(E,X) to again create an (RBS+BP1) + (RBS+BP2) a...)
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*Cut parts for A70-A72
 
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**(RBS+BP) assemblies were cut E,S
 
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**B0015 was cut E,X
 
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**>Standard assembly
 
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*Also, used old cuts of A58(E,S) and A59(E,X) to again create an (RBS+BP1) + (RBS+BP2) assembly
 
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*Used newer B0015(E,X) and older I715039(E,S) to attached terminator to PRE RFP
 
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*Ligated, heat-inactivated and transformed with 2ul of DNA into 25ul of cells
 
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*Plated
 
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[[Team:Virginia/Notebook|Back to Notebook]]
 

Latest revision as of 04:21, 8 September 2008

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