Wiki/Team:Warsaw/igem notebook.htm

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Gallery Bricks Notebook Team Project Home

Table of contents

 

Preparation of one-armed bandit vectors

One-armed bandit tests

Preparation of vectors for 'hunter' part of 'hunter and prey'

Preparation of vectors for 'prey' part of 'hunter and prey'

Purification of prey proteins

'Hunter and prey' tests

Preparation of BioBricks

Other activities



Protocols

Primers

Vectors

JavaScript tools developed to make work with iGEM notebook more comfortable

Strains

Rosetta(DE3)pLysS

F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)

TOP10 (Invitrogen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-

NR9458 (IBB collection; in notebook called mutD5)

ara thi Δ pro lac mutD5 zaf-13 ::Tn10

GM2163

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2

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