Performed PCR cleanup and reactions for sequencing.
Submitted for sequencing.
Sequencing results received same day. All colonies perfect matches with new primers. Success!
Ligation product transformed into E.coli.
Second His-tag purification resulted again with no protein. Now it is believed to be too small an amount for the nanodrop to measure.
Team Fungus:
Remade assay solutions due to contaminants.
Re-ran Azure B assay on 96 well plate.
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