Wisconsin: Lignin Project/10 July 2008

From 2008.igem.org

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== Team Sorbitol ==
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<div style="background: #000; padding: 10px;">
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Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
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<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
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Started the following cultures:
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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|'''Team Sorbitol:'''<br>
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DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon
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Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.<br>
-
 
+
Started the following cultures:<br>
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DH5a-pBAD30-srlD: from colony 2 to freeze down for back up
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DH5a-PBAD30 : to harvest the plasmid to clone out the ''srl'' operon<br>
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DH5a-pBAD30-srlD: from colony 2 to freeze down for back up<br>
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DH5a-pBAD18 - to insert ''srld'' and the operon into.
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DH5a-pBAD18 - to insert ''srld'' and the operon into MG1655, JW3890, RL257<br>
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This was done to make chemically competent<br>
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|}
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MG1655, JW3890, RL257 - all to make chemically competent
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Revision as of 15:42, 8 August 2008

Igemwibanner.gif
Team Sorbitol:

Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
Started the following cultures:
DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon
DH5a-pBAD30-srlD: from colony 2 to freeze down for back up
DH5a-pBAD18 - to insert srld and the operon into MG1655, JW3890, RL257
This was done to make chemically competent