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Wisconsin: Lignin Project/17 June 2008
From 2008.igem.org
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| - | == Team Sorbitol | + | |'''Team Sorbitol:''' |
Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. | Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. | ||
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However when run on a gel no bands were found. | However when run on a gel no bands were found. | ||
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| + | '''Team Fungus:''' <br> | ||
| + | Received primers <br> | ||
| + | Diluted primers <br> | ||
| + | Ran TAQ PCR reaction with Carbon-limited cDNA <br> | ||
| + | Ran PCR product on 1% agrose gel; only ladder visible <br> | ||
| + | |} | ||
Revision as of 15:15, 8 August 2008
| Team Sorbitol:
Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells. Used this DNA as a template for 2 PCR reactions One reaction was to clone out srlD the other was to clone the srl operon. However when run on a gel no bands were found. Team Fungus: |
