Wisconsin: Lignin Project/17 June 2008

From 2008.igem.org

Revision as of 15:15, 8 August 2008 by TPeelen (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Igemwibanner.gif
Team Sorbitol:

Harvested the chromosomal DNA from the overnight MG1655 cultures by boiling the cells.
Used this DNA as a template for 2 PCR reactions.
One reaction was to clone out srlD the other was to clone the srl operon.
However when run on a gel no bands were found.

Team Fungus:
Received primers
Diluted primers
Ran TAQ PCR reaction with Carbon-limited cDNA
Ran PCR product on 1% agrose gel; only ladder visible