Wisconsin: Lignin Project/23 June 2008

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Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA using Herculase II polymerase<br>
Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA using Herculase II polymerase<br>
Ran PCR products through 0.9% agrose gel at 100V<br>
Ran PCR products through 0.9% agrose gel at 100V<br>
-
-Bands present for both at about 1.1kb <br>
+
Bands present for both at about 1.1kb <br>
Grew overnight culture of pET28a <br>
Grew overnight culture of pET28a <br>

Revision as of 15:21, 8 August 2008

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Team Sorbitol:

Decided to use Herculase instead of TAQ in the PCR reactions (Herculase has proofreading ablitity)
Ran a new PCR reaction using the specific volumes designated by the Herculase designers.
No Bands formed.

Team Fungus:
Ran a touchdown PCR of Carbon and Nitrogen-limited cDNA using Herculase II polymerase
Ran PCR products through 0.9% agrose gel at 100V
Bands present for both at about 1.1kb

Grew overnight culture of pET28a
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