Wisconsin: Lignin Project/26 June 2008

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The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.<br>
The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.<br>
Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18.<br>
Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18.<br>
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Found there was once a commercial kit for quantifying sorbitol (Boehringer), however it was now not available in the market.<br>
'''Team Fungus:''' <br>
'''Team Fungus:''' <br>

Revision as of 02:48, 29 October 2008

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Team Sorbitol:

Found that creating fresh proteinase K solution every time will work better.
Designed and set up a gradiant PCR (using Herculase).
Several successful bands for srlD were obtained.
The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.
Also designed sequencing primers to test for srlD in both pBAD30 and pBAD18.
Found there was once a commercial kit for quantifying sorbitol (Boehringer), however it was now not available in the market.

Team Fungus:
Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1
Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight

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