Team:Hawaii/Notebook/2008-07-31
From 2008.igem.org
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Things we did today
Wetlab work
Ligation of PCR products
- Grace & Krystle
- Ligated nir+rbs at 6:1 and 3:1 insert to vector concentrations (we didn't clean up RE digest so GP suggests higher vector concentration)
- Ligated GFP+tt and GFPf+tt at 6:1 insert to vector concentrations (didn't have enough vector to do 3:1)
- Ligated J33207+pRL1383a at 2:1 and 4:1 insert to vector concentrations
Transformed DB3.1 with ligated products
- Grace
Ran a gel to check 7/30 gel
- Krystle
- Loaded 2 μl PCR product into each well
- PCR tubes likely mislabeled
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson