Team:Hawaii/Notebook/2008-07-31

From 2008.igem.org

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Things we did today

Wetlab work

Ligation of PCR products

Grace & Krystle
  • Ligated nir+rbs at 6:1 and 3:1 insert to vector concentrations (we didn't clean up RE digest so GP suggests higher vector concentration)
  • Ligated GFP+tt and GFPf+tt at 6:1 insert to vector concentrations (didn't have enough vector to do 3:1)
  • Ligated J33207+pRL1383a at 2:1 and 4:1 insert to vector concentrations

Transformed DB3.1 with ligated products

Grace

Ran a gel to check 7/30 gel

Krystle
  • Loaded 2 μl PCR product into each well
  • PCR tubes likely mislabeled

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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