Team:Harvard

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<font size=1>Our project sought to combine the detecting capabilities of bacteria with the speed and ubiquity of electricity by creating an inducible system in Shewanella oneidensis MR-1 with an electrical output, allowing for the direct integration of this biosensor with electrical circuits via microbial fuel cells.</font>
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Shewanella oneidensis MR-1 <br>
(fondly referred to as Shewie)<br>
is a metabolically versatile, <br>
and genetically tractable, gram-<br>
negative facultative anaerobe which under <br>
anaerobic conditions reduces a number of electron <br>
acceptors.  This ability can be harnessed by <br>
microbial fuel cells to produce an electric current.
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The broad goal of our project was to engineer S. Oneidensis to produce a detectable change in electric current in response to some environmental stimulus. In order to observe such a reaction, our first task was to design an environment capable of housing bacteria and measuring current production. The answer? Microbial fuel cells.
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