Genetic Circuit

=Part II Genetic Circuit=
Under Matlab Simbiology Toolbox, we demonstrate all the reactions listed below.

SimBiology Reaction Array

   Index:    Reaction:
   1         DNA.lacO_hAID_lexA + Pro.LacI <-> DNA.[lacO:LacI]
   2         DNA.lacO_hAID_lexA -> mRNA.lacO_hAID_lexA + DNA.lacO_hAID_lexA
   3         mRNA.lacO_hAID_lexA -> null
   4         mRNA.lacO_hAID_lexA -> Pro.hAID_LexA
   5         Pro.hAID_LexA + DNA.DeGal4_lexO <-> DNA.[hAID_LexA:DeGal4_lexO]
   6         Pro.hAID_LexA -> null
   7         DNA.Gal4_LexO -> mRNA.Gal4_LexO + DNA.Gal4_LexO
   8         mRNA.Gal4_LexO -> Pro.Gal4
   9         Pro.Gal4 + DNA.UAS_his_lacZ_lacI -> DNA.[Gal4:UAS_his_lacZ_lacI]
   10        DNA.[Gal4:UAS_his_lacZ_lacI] -> mRNA.his_lacZ_LacI
   11        mRNA.his_lacZ_LacI -> Pro.LacI + Pro.His + Pro.LacZ
   12        mRNA.Gal4_LexO -> null
   13        mRNA.his_lacZ_LacI -> null
   14        Pro.LacI -> null
   15        Pro.Gal4 -> null
   16        DNA.[hAID_LexA:Gal4_lexO] -> Pro.hAID_LexA + DNA.Gal4_LexO
   17        DNA.[hAID_LexA:DeGal4_lexO] -> DNA.[hAID_LexA:Gal4_lexO]
   18        Pro.His -> null
   19        Pro.LacZ -> null

The diagram of the network is shown as well.
[[Image:Peking hAID final.jpg]]
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To simplify the model, we combine the transcription and replication to one process of DNA expression. Also introduce the Hill-equation to demonstrate the function of repression and activation of the transcriptional factors. Other technical procedure introduced as well, we simplify the model further. The simplified model is as below.
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[[Image:Peking hAID simplified.jpg]]
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