Team:Chiba/Sender experiments/Senders(JW1908) T9002(JW1908)

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==Results and Discussion==
===Reaction temparature:37°C,09/12===
====Sender culture：1000&mu;L,Receiver culture:1000&mu;L====
[[Image:Chiba_talks_JW1908_37_RS1_0912_01.gif‎‎|thumb|left|'''Fig.1'''　　<br>12/09.E.coli strain,senders:JW1908,BBa_T9002:JW1908,Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
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====Sender culture：100&mu;L,Receiver culture：1000&mu;L====

[[Image:Chiba_talks_JW1908_37_RS2_0912_01.gif‎|thumb|left|'''Fig.2'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,37&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
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*Fig. 1,Fig. 2

Green fluorescence intensity didn't increase in the culture containing BBa_K0840010(plac+CinI+LVA).We thought BBa_K084010 didn't work properly or LuxR gene didn't interact with signal molecules synthesized by BBa_K084010.
Others responded at the same time(4 hours after induction).Final fluorescence intensity differd.

===Reaction temparature:37°C===
====Sender culture：500&mu;Lm,Receiver culture：500&mu;L====
[[Image:Chiba_communication_JW1908_37_RS1_01.gif‎|thumb|left|'''Fig.3'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,37&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
[[Image:Chiba_talks_JW1908_37_RS1_02.gif‎|thumb|left|'''Fig.4'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,37&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
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*Fig. 3

Response time and final fluorescence intensity showed no significant difference.
We thought AHL concentration was quickly reached the threshold concentration.The fluorescence intensity increased as gfp maturured.

*Fig. 4

No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

===Reaction temparature:30°C===
====Sender culture：500&mu;L,Receiver culture：500&mu;L====
[[Image:Chiba_talks_JW1908_30_RS1_01.gif|thumb|left|'''Fig.5'''　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
[[Image:Chiba_talks_JW1908_30_RS1_02.gif|thumb|left|'''Fig.6'''　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]

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*Fig. 6
No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

====Sender culture：100&mu;L,Receiver culture：1000&mu;L====
[[Image:Chiba_talks_JW1908_30_RS2_01.gif|thumb|left|'''Fig.7'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
[[Image:Chiba_talks_JW1908_30_RS2_02.gif|thumb|left|'''Fig.8'''　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
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No significant difference in fluorescence intensity between the culture containing [http://partsregistry.org/Part:BBa_K084007 BBa_K084007](plac+RhlI) gene transformed cells and the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008](plac+RhlI(LVA)) gene transformed cells.We thought that the rate of AHL synthesis by each autoinducer synthase was much faster than the rate of its degradation by protease.

====Sender culture：10&mu;L,Receiver culture：1000&mu;L====
[[Image:Chiba_talks_JW1908_30_RS3_02.gif|thumb|left|'''Fig.9'''　　<br>E.coli strain,senders:JW1908,BBa_T9002:JW1908,30&deg;C.All measurements are averages from three replicate cultures with error bars representing standard deviations.]]
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The final fluorescence intensity of the culture containing [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] transformed cells which express LVA-tagged RhlI protein was lower than the culture containing cells which express untagged RhlI protein.It shows that LVA-tagged RhlI protein was degraded by LVA-specific protase.However,fluorescence intensity of both cultures increased at the same time.We thought it was because the AHL concentration was quickly reached the threshold concentration and cells began to express gfp.After 2 hours,fluorescence intensity started to increase.  

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[[Team:Chiba/Project/Experiments:Sender_Crosstalk|>Back to Sender experiment and result]]