Team:UC Berkeley/Resources/MMCalculator

==Bing's Master Mix Calcualtor Version 0.2a==
'''All volumes are given in uL. The calculator will give you enough master mix for one extra reaction.'''

<html>
<head>
<script type="text/javascript">
function calcVolumes() {
	reaction = document.getElementById("rxnType").selectedIndex;
	out = document.getElementById("output");
	out.value = "";
	num = parseInt(document.getElementById("numRxns").value) + 1;
	switch(reaction)
	{
	case 0:
		out.value += "Regular PCR w/o DMSO Master Mix for " + num + " reactions (25 uL scale):\n";
		out.value += "Water: " + (20.375*num) + "\n";
		out.value += "10 mM dNTP: " + (.5*num) + "\n";
		out.value += "10x Expand Buffer \"2\": " + (2.5*num) + "\n";
		out.value += "Expand Polymerase \"1\": " + (.375*num) + "\n";
		out.value += "Aliquot 23.75 uL\n";
		break;
	case 1:
		out.value += "Regular PCR w/ DMSO Master Mix for " + num + " reactions:\n";
		out.value += "Water: " + (17.875*num) + "\n";
		out.value += "DMSO: " + (2.5*num) + "\n";
		out.value += "10 mM dNTP: " + (.5*num) + "\n";
		out.value += "10x Expand Buffer \"2\": " + (2.5*num) + "\n";
		out.value += "Expand Polymerase \"1\": " + (.375*num) + "\n";
		out.value += "Aliquot 23.75 uL\n";
		break;
	case 2:
		out.value += "Wobble PCR Master Mix for " + num + " reactions:\n";
		out.value += "Water: " + (40*num) + "\n";
		out.value += "MgCl (10 mM): " + (1.5*num) + "\n";
		out.value += "10 mM dNTP: " + (1*num) + "\n";
		out.value += "10x Taq Buffer: " + (5*num) + "\n";
		out.value += "Taq Polymerase: " + (.5*num) + "\n";
		out.value += "Aliquot 48 uL\n";
		break;
	case 3:
		out.value += "Colony PCR Master Mix for " + num + " reactions:\n";
		out.value += "Water: " + (16*num) + "\n";
		out.value += "MgCl (50 mM): " + (.6*num) + "\n";
		out.value += "10 mM dNTP: " + (.4*num) + "\n";
		out.value += "10x Taq Buffer: " + (2*num) + "\n";
		out.value += "Taq Polymerase: " + (.2*num) + "\n";
		out.value += "ca998 Oligo(10 mM): " + (.4*num) + "\n";
		out.value += "g00101 Oligo(10 mM): " + (.4*num) + "\n";
		out.value += "Aliquot 20 uL\n";
		break;
	case 4:
		out.value += "Digestion Master Mix for " + num + " reactions:\n";
		out.value += "This is assuming that you are using 20 uL of your DNA sample!!!!\n";
		out.value += "Water: " + (23*num) + "\n";
		out.value += "NEB2: " + (5*num) + "\n";
		out.value += "EcoRI: " + (1*num) + "\n";
		out.value += "BamHI: " + (1*num) + "\n";
		out.value += "Aliquot 30 uL\n";
		break;
	case 5:
		out.value += "Ligation Master Mix for " + num + " reactions:\n";
		out.value += "Water: " + (6.5*num) + "\n";
		out.value += "Ligation Buffer: " + (1*num) + "\n";
		out.value += "T4 Ligase: " + (.5*num) + "\n";
		out.value += "Digested pBca1256 Vector: " + (1*num) + "\n";
		out.value += "Aliquot 9 uL\n";
		break;
	case 6:
		out.value += "Regular PCR w/o DMSO w/ oligos, 50 uL Scale Master Mix for " + num + " reactions:\n";
		out.value += "Water: " + (40.75*num) + "\n";
		out.value += "10 mM dNTP: " + (1*num) + "\n";
		out.value += "10x Expand Buffer \"2\": " + (5*num) + "\n";
		out.value += "Oligo #1: " + (1*num) + "\n";
		out.value += "Oligo #2: " + (1*num) + "\n";
		out.value += "Expand Polymerase \"1\": " + (.75*num) + "\n";
		out.value += "Aliquot 49.5 uL\n";
		break;
	case 7:
		out.value += "Basic Part Plasmid Digestion Master Mix for " + num + " reactions:\n";
		out.value += "Water: " + (21*num) + "\n";
		out.value += "NEB2 Buffer: " + (3*num) + "\n";
		out.value += "EcoRI: " + (.5*num) + "\n";
		out.value += "BamHI: " + (.5*num) + "\n";
		out.value += "Aliquot 49.75 uL\n";
		break;
	}
}
</script>
</head>

<body>
<form>
	Select Reaction:
	<select id="rxnType">
		<option>Regular PCR w/o DMSO</option>
		<option>Regular PCR w/ DMSO</option>
		<option>Wobble PCR</option>
		<option>Colony PCR</option>
		<option>Digestion</option>
		<option>Ligation</option>
		<option>Regular PCR w/o DMSO, 50 uL scale</option>
		<option>Basic Part Plasmid Digest</option>
	</select>
	<br>
	<p>Number of Reactions: <input type="text" id="numRxns"> </input> </p> <br>
	<input type="button" onclick="calcVolumes()" value="Calculate"></input>
</form>
<textarea value="hello" id="output" cols="10" rows="15"> </textarea>
</body>
</html>

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