PCR products

<html>
<head>
<title>Edinburgh iGem 2008</title>
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
//Created: May 16th, 07'

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	//No need to edit beyond here////////////////////////
	dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
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			this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
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<style>
.ddcolortabs{
padding: 0;
width: 100%;
background: transparent;
voice-family: "\"}\"";
voice-family: inherit;
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margin:0;
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margin:0 2px 0 0;
padding:0;
text-transform:uppercase;
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.ddcolortabs a{
float:left;
color: white;
background: black url(media/color_tabs_left.gif) no-repeat left top;
margin:0 2px 0 0;
padding:0 0 1px 3px;
text-decoration:none;
letter-spacing: 1px;
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.ddcolortabs a span{
float:left;
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
padding: 4px 8px 2px 7px;
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.ddcolortabs a span{
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.ddcolortabs a:hover{
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.ddcolortabs a:hover span{
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
background-color: #591f20;
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.ddcolortabsline{
clear: both;
padding: 0;
width: 100%;
height: 8px;
line-height: 8px;
background: black;
border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
}

/* ######### Style for Drop Down Menu ######### */

.dropmenudiv_a{
position:absolute;
top: 0;
border: 1px solid black; /*THEME CHANGE HERE*/
border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
border-bottom-width: 0;
font:normal 12px Arial;
line-height:18px;
z-index:100;
background-color: white;
width: 200px;
visibility: hidden;
}


.dropmenudiv_a a{
width: auto;
display: block;
text-indent: 5px;
border-top: 0 solid #678b3f;
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
padding: 2px 0;
text-decoration: none;
color: black;
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* html .dropmenudiv_a a{ /*IE only hack*/
width: 100%;
}

.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
background-color: #8a3c3d;
color: white;
}
</style>
</head>

<!-- CSS for Drop Down Tabs Menu #1 -->
<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />

<div id="colortab" class="ddcolortabs">
<ul>
<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>	
<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>	
</ul>
</div>
<div class="ddcolortabsline">&nbsp;</div>
<div id="dropmenu1_a" class="dropmenudiv_a">
<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
</div>
<script type="text/javascript">
//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
tabdropdown.init("colortab", 3)
</script>
</html>

'''P1:''' ''dxs'' from ''Eschaerichia coli'' JM109 (or K12?)<br />
'''P2:''' ''appY'' from ''E. coli'' JM109 (or K12?)<br />
'''P3:''' ''glgC'' from ''E. coli'' JM109 (or K12?)<br />
'''P4:''' fusion PCR for BABEL1+''glgC''<br />
'''P5:''' fusion PCR for BABEL2+''glgC''<br />
'''P6:''' mutagenic PCR to remove EcoRI site 1 from ''glgC''<br />
'''P7:''' mutagenic PCR to remove EcoRI site 2 from ''glgC''<br />
'''P8:''' ''crtB'' (from ''Pantoea ananatis'' cells)<br />
'''P9:''' ''crtI'' (from Douglas's maxiprep of the mutated ''crtIB'' plasmid)<br />
'''P10:''' ''crtY'' (from ''P. ananatis'' cells). Failed.<br />
'''P11:''' fusion PCR for rbs+''dxs''. Failed.<br />
'''P12:''' repeat fusion PCR for rbs+''dxs'', using L11 and primers rbs2fclon1+pSB1A2insr1.<br />
'''P13 and P14:''' MABEL mutation of site 1 on ''glgC'' mutant clones M19 and M22.<br />
'''P15:''' fusion PCR for rbs+''appY''<br />
'''P16, P17, P18 and P19:''' first attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
'''P20, P21, P22 and P23:''' second attempts at ''cenA'', ''cenB'', ''cenC'' and ''cex''.<br />
'''P24, P25:''' repeat PCR for ''cenA'' and ''cex'' using Pfu. Failed.<br />
'''P26:''' Positive control PCR with primers fd1 and rd1 to amplify 16S rRNA gene ''rrnB'' from ''Cellulomonas fimi''.<br />
'''P27:''' ''crtY'' from ''P. ananatis'' cells. - Failed<br />
'''P28, P29:''' ''crtB'' and ''crtI'' from L18 and L19.<br />
'''P30:''' Repeat positive control ''rrnB'' from ''C. fimi'', annealing 1 minute.<br />
'''P31:''' pZntA promoter<br />
'''P32-P35:''' repeat PCR for ''cenA'', ''cenB'', ''cenC'' and ''cex'', annealing 1 minute. Failed.<br />
'''P36:''' M43 (''glgC''-mut1,2) with primers glgCf2/glgCr2 (25.07.08: AM)<br />
'''P37:''' ''crtY'' from ''P. ananatis''. (25.07.08: AM) - Failed<br />
'''P38~P41:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from heat-killed cell suspension.<br />
'''P42~P45:''' ''cenA'', ''cenB'', ''cenC'' and ''cex'' from 'impure' DNA solution.<br />
'''P46~P47:''' ''cenA'' and ''cex'' from 'impure' DNA solution, annealing 65C. Failed.<br />
'''P48:''' ''crtY'' (new primer mixture) (previously P38a) (30.07.08: CF) - Failed<br />
'''P49:''' Recreation of P12 (rbs+''dxs'') (previously P39a) (30.07.08: CF)<br />
'''P50:''' third mutagenesis of ''glgc''-mut1,2 (04.08.08: YAN)<br />
'''P51:''' pCstA from ''E. coli'' cells (06.08.08: CF) - Failed<br />
'''P52:''' ''cenA'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
'''P53:''' ''cex'' from heat killed ''C. fimi'' (07/08/08: YAN, AM) - Failed<br />
'''P54:''' pCstA from ''E. coli'' cells - Purified (07.08.08: CF)<br />