Team:BCCS-Bristol/Protocols-Transformation with heat shock

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==Transformation with heat shock==

#	Thaw the chemical competent bacteria (50 µl ''E. coli'' DH5α) on ice
#	Add the DNA by pipetting it directly in the middle of the bacteria solution und mix by stirring gently with the pipette tip
#	Incubate 20 min on ice
#	Heat shock: incubate 60 s at 42°C in a water bath
#	Incubate 2 min on ice
#	Add 250 µl SOC broth (stored in the freezer; thaw before and check for no contamination!)
#	Incubate for 1 h at 37°C 225 rpm
#	Plate 50 µl and 100 µl on two LB plates with antibiotics
#	Incubate the plates at 37°C overnight. Store the remaining bacteria in the fridge.