Team:Caltech/Protocols/FimE

{{Caltech_iGEM_08|
Content=

<div style="font-size:18pt;">
<font face="verdana" style="color:#CC3300">FimE Inversion Assay</font></div>
__NOTOC__
<br>
# Grow 5mL LB cultures with appropriate antibiotics overnight. 
# Backdilute culture 1:100 into fresh 5mL LB with appropriate antibiotics.
# When the cultures reach 0.4 OD at 600nm, add arabinose and grow at 37C for 1 hour.
# Wash and resuspend cells in phosphate buffer solution.
# Measure GFP fluorescence on plate reader.
}}