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==Making Overnight Cultures from Agar Colonies== * Pipette 10mL LB into red-capped culture tubes or conical shake flasks. * Using an autoclaved toothpick, gently touch the point to a single colony. * Drop the toothpick point down into the LB. * Incubate overnight at 37°C whilst shaking. Back to [[Team:Newcastle University/Notebook]]

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