IGEM:Cambridge/2008/Notebook/Voltage/Future Work

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=Future Work=

==Better K+ uptake==
If a stronger K+ uptake could be made then in principal cells could be grown in the same medium as testing was done. This would potentially allow cells to be grown as a surface over an agar plate and still act to produce action potentials, thereby acting as neurones.
*Up regulate KDP constitutively
*Find K+ tolerant strains
*Check maximum trans-membrane potential

==Move to [[Team:Cambridge/Bacillus|''Bacillus'']]==
Need to make GluR0 ''Bacillus'' compatible
*Codon bias already favourable for ''Bacillus''
*Need to put different promoter and RBS
*Integrate into genome?

==Voltage Gated Potassium Channel==
To get self propagating signal we need to find and construct a voltage gated potassium channel.
*Relatively rare in Prokaryotes
*Some available although not well characterised, such as Kch
*Overexpress and see if it improves output.

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