Team:ESBS-Strasbourg/Transformation Heat shock

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Protocol is a mixture of the IGEM standard protocol and the protocol used in the local lab<br>
* ligation or 10 ng plasmid
* add 50-100 µL of competent bacteria
* take on ice for 30 min
* heat shock at 42 °C for 2 min
* add 300 µL LB and incubated at 37°C for 45 min
* plate
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