Team:Edinburgh/Project/iGEM 2008 Labwork Summary/Our Primers

== Our Primers ==


'''This is the current situation with regard to primers:'''

=== Primer sequences: ===
Cellulomonas fimi endogluconase A (cenA)
Suitable BioBrick primers should be:

Forward:    CGT gaattc gcggccgc t tctagATG TCC ACC CGC AGA ACC 
Reverse:    CGT t actagt a TTA TTA CCA CCT GGC GTT GC   




Cellulomonas fimi endogluconase B (cenB)
Suitable BioBrick primers should be:

Forward:    CGT gaattc gcggccgc t tctagATG CTC CGC CAA GTC CC 
Reverse:    CGT t actagt a TTA TTA GTT CGT CGG GAT G   




Cellulomonas fimi endogluconase C (cenC)
Suitable BioBrick primers should be:

Forward:    CGT t tctagATG GTT TCT CGC AGG TC  
Reverse:    CGT ctgcag cggccgc t actagt a TTA TTA GCT GCG CGG ACG C   




Cellulomonas fimi exogluconase (cex)
Suitable BioBrick primers should be:

Forward:    CGT t tctagATG CCT AGG ACC ACG CC  
Reverse:    CGT ctgcag cggccgc t actagt a TTA TTA GCC GAC CGT GCA GG   




E. coli glucose-1-phosphate adenylyltransferase (glgC)
Suitable mutant primers are:

'''to remove the first forbidden EcoRI site:''' 


Forward:    tgttgaaaaacctgctaacc - 54°C 
Reverse:    aattcgataattttatcgttc -52°C  

''' to remove the second forbidden EcoRI site:'''

Forward:    ctcattctgcaacattgattcc - 54°C 
Reverse:    ttcacgcgaacgcgcg - 54°C  

'''to cause 336:Gly->Asp:'''

Forward:    acggttgtgtgatctccg - 54°C 
Reverse:    cggaaaccagtgagttaag- 56°C  

 
Arabidopsis thaliana isoamylase 1 (isa1)
Suitable BioBrick primers should be:

Forward:    CGT t tctagATG GAT GCA ATC AAA TGC AG - 56ºC 
Reverse:    CGT ctgcag cggccgc t actagt aTTA TTA GGG GTC TTT AAT TGG TG - 58ºC  




Arabidopsis thaliana isoamylase 2 (isa2)
Suitable BioBrick primers should be:

Forward:    CGT ttctagATG GCT GCG TGG TCA CC - 56ºC 
Reverse:    CGT ctgcag cggccgc t actagt a TTA TTA AGC GGT AGT ATT GAT GG - 54ºC  




Arabidopsis thaliana granule bound starch synthase 1 (GBS1)
Suitable BioBrick primers should be:

Forward:    CGT gaattc gcggccgc t tctagATG GCA ACT GTG ACT GCT - 54ºC 
Reverse:    CGT t actagt a TTA TTA CGG CGT CGC TAC G - 52ºC