Team:Hawaii/Protocols/oligo hybridization

==Hybridization of Parts==
===Protocol===
#Mix: 
#:3 μl 100 µM sense oligo 
#:3 μl 100 µM anti-sense oligo 
#:3 μl 10 x PNK (polynucleotide kinase) buffer 
#:2 μl 10mM ATP 
#:2 μl T4 polynucleotide kinase (PNK) 
#:17 μl distilled water 
#:Total volume = 30 μl 
#Incubate at 37C for 1.5 hours.
#Add 4 μl 0.5 M NaCl. 
#Heated mixture in PCR machine to 97C for 2 min. Cooled mixture at each degree between 80 and 96 for 2 min (Tm range of oligos). Cooled mixture at each degree between 25 and 79 for 1 min.
 
:::''Reference: Pam Silver Lab. [http://openwetware.org/wiki/Silver:_Oligonucleotide_Inserts| Oligonucleotide Inserts.]''