Team:UNIPV-Pavia/Protocols/GelExtraction

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<h1>The protocols we used</h1>

*[[Team:UNIPV-Pavia/Protocols/Lb|LB medium preparation]]
*[[Team:UNIPV-Pavia/Protocols/Resuspension|Plasmid resuspension from IGEM paper spots]]
*[[Team:UNIPV-Pavia/Protocols/Transformation|Transformation]]
*[[Team:UNIPV-Pavia/Protocols/PlasmidExtraction|Plasmid extraction]]
*[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]]
*[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]]
*[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]]
*[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]]
*[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]]
*[[Team:UNIPV-Pavia/Protocols/Pcr|PCR]]


<h1>DNA gel extraction</h1>
''(Estimated time: 1 hour and 30 min)''
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<br>
'''Materials needed:'''
*'''Agarose gel with well-separated, sharp bands'''
*'''Scalpel and tweezers to cut gel''' (they have to be cleaned in ddH2O after every cut)
*'''Roche Agarose Gel DNA Extraction Kit'''
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*Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol]
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