Team:University of Lethbridge/Notebook/Project4September

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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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===September 7, 2008===
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====Selina====
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Objective: Create glycerol stocks of the xylE plasmid for future use.
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-------------------
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Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.
 +
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Followed manufacturer's (Invitrogen) 'high-yield' protocol.
 +
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Plated 100 uL on LB + Amp and incubated overnight at 37C.
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===September 8, 2008===
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====Selina====
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Checked xylE transformation plates for colonies - success!
 +
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Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours.
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====Christa, Selina====
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Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C.

Latest revision as of 02:23, 30 October 2008

Back to The University of Lethbridge Main Notebook

Contents

September 7, 2008

Selina

Objective: Create glycerol stocks of the xylE plasmid for future use.

Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.

Followed manufacturer's (Invitrogen) 'high-yield' protocol.

Plated 100 uL on LB + Amp and incubated overnight at 37C.


September 8, 2008

Selina

Checked xylE transformation plates for colonies - success!

Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours.

Christa, Selina

Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C.

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