Team:University of Lethbridge/Notebook/Project4September

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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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===September 7, 2008===
===September 7, 2008===
====Selina====
====Selina====
 +
Objective: Create glycerol stocks of the xylE plasmid for future use.
 +
 +
-------------------
Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.
Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.
-
Followed manufacturer's (NEB) 'high-yield' protocol.
+
Followed manufacturer's (Invitrogen) 'high-yield' protocol.
Plated 100 uL on LB + Amp and incubated overnight at 37C.
Plated 100 uL on LB + Amp and incubated overnight at 37C.
 +
 +
 +
===September 8, 2008===
 +
====Selina====
 +
 +
Checked xylE transformation plates for colonies - success!
 +
 +
Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours.
 +
 +
====Christa, Selina====
 +
 +
Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C.

Latest revision as of 02:23, 30 October 2008

Back to The University of Lethbridge Main Notebook

Contents

September 7, 2008

Selina

Objective: Create glycerol stocks of the xylE plasmid for future use.

Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.

Followed manufacturer's (Invitrogen) 'high-yield' protocol.

Plated 100 uL on LB + Amp and incubated overnight at 37C.


September 8, 2008

Selina

Checked xylE transformation plates for colonies - success!

Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours.

Christa, Selina

Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C.

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