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Team:University of Lethbridge/Notebook/Project4September
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===September 7, 2008=== | ===September 7, 2008=== | ||
====Selina==== | ====Selina==== | ||
| - | Objective: Create glycerol stocks of the xylE | + | Objective: Create glycerol stocks of the xylE plasmid for future use. |
| + | ------------------- | ||
Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells. | Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells. | ||
| - | Followed manufacturer's ( | + | Followed manufacturer's (Invitrogen) 'high-yield' protocol. |
Plated 100 uL on LB + Amp and incubated overnight at 37C. | Plated 100 uL on LB + Amp and incubated overnight at 37C. | ||
| Line 18: | Line 19: | ||
Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours. | Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours. | ||
| - | |||
====Christa, Selina==== | ====Christa, Selina==== | ||
Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C. | Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C. | ||
Latest revision as of 02:23, 30 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
September 7, 2008
Selina
Objective: Create glycerol stocks of the xylE plasmid for future use.
Set up transformation of xylE pVL1392 plasmid (50 pg) with 25 uL E. coli DH5a cells.
Followed manufacturer's (Invitrogen) 'high-yield' protocol.
Plated 100 uL on LB + Amp and incubated overnight at 37C.
September 8, 2008
Selina
Checked xylE transformation plates for colonies - success!
Inoculated one 5 mL LB + Amp culture tube and incubated at 37 C for 4.5 hours.
Christa, Selina
Created 3 glycerol stocks of E. coli DH5a xylE pVL1392 transformants and placed in -80C.
