Team:Cambridge

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     <a href="https://2008.igem.org/Team:Cambridge/Signalling" class="noborder"><img src="http://openwetware.org/images/9/9d/Signalling_button.gif" alt="Signalling" width="250"></a>
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     <a href="https://2008.igem.org/Team:Cambridge/Signalling" class="noborder"><img src="http://openwetware.org/images/9/9d/Signalling_button.gif" alt="Signalling" width="250" style="padding: 3px 0px;"></a>
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     <a href="https://2008.igem.org/Team:Cambridge/Bacillus"><img src="http://openwetware.org/images/8/8e/Bacillus_button.gif" alt="Bacillus" width="250"></a>
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     <a href="https://2008.igem.org/Team:Cambridge/Bacillus"><img src="http://openwetware.org/images/8/8e/Bacillus_button.gif" alt="Bacillus" width="250" style="padding: 3px 0px;"></a>
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     <a href="https://2008.igem.org/Team:Cambridge/Voltage"><img src="http://openwetware.org/images/7/74/Voltage_button.gif" alt="Voltage" width="250"></a>
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     <a href="https://2008.igem.org/Team:Cambridge/Voltage"><img src="http://openwetware.org/images/7/74/Voltage_button.gif" alt="Voltage" width="250" style="padding: 3px;"></a>
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     <a href="https://2008.igem.org/Team:Cambridge/Modelling"><img src="http://openwetware.org/images/9/91/Modelling_button.gif" alt="Modelling" width="250"></a>
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     <h1>Overview</h1>
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     <b>Since the emergence of Synthetic Biology</b>, bacteria have been engineered to perform a wide variety of simple tasks. They can be made to express proteins, respond to their environment and communicate primitively with each other. Presently, a key goal for the field is to create a communicating, organised and differentiated population of bacteria that can be considered a multicellular organism, capable of performing even more complex tasks. To realize this goal requires the development of systems for rapid, robust communication and self-organised differentiation. Our project sets the foundation for future research in engineered multi-cellularity by pursuing electrical and peptide signalling, and cellular self-differentiation through spontaneous spatial patterning.
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     <b>Since the emergence of Synthetic Biology</b>, bacteria have been engineered to perform a wide variety of simple tasks. They can be made to express proteins, respond to their environment and communicate primitively with each other. Presently, a key goal for the field is to create a communicating, organised and differentiated population of bacteria that can be considered a multicellular organism, capable of performing even more complex tasks. The ultimate goal for this line of research would be to mimic a brain, the most complex structure in the universe. To realize this goal requires the development of systems for rapid, robust communication and self-organised differentiation. <br /> <b> Our project sets the foundation for future research in engineered multi-cellularity by pursuing electrical and peptide signalling, and cellular self-differentiation through spontaneous spatial patterning.</b>
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          <h1>Voltage</h1>
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In order to simulate neural activity in bacteria, a mechanism resembling a synapse is necessary. At the synapse, neurotransmitter molecules are released from the presynaptic plasma membrane. The neurotransmitter diffuses through the synaptic cleft and binds to chemical receptor molecules on the membrane of the postsynaptic cell. These receptors cause ion channels to open so that ions rush out, changing the transmembrane potential. Attempting to mimic this in a prokaryotic system is particularly attractive as, in a more general sense, it provides an interface between chemical or biological and electrical systems. <br /><b> Using the amino acid glutamate as our 'neurotransmitter', we have successfully demonstrated a voltage response in bacterial cells. </b> <a href="https://2008.igem.org/Team:Cambridge/Voltage"> Read on...</a>
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===MIGRATION: Note to Cambridge iGEMers===
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We are manually transferring all wiki text and images from OWW to this page. Please check the email you were sent for your particular subsection. Please transfer images as well as text. Transferring images requires downloading them to your computer and re-uploading them to this website.  
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          <h1>Signalling</h1>
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Using peptide-based signalling systems from gram-positive bacteria, we have laid the foundations for a self-organising biological system, capable of expressing spatial patterns of GFP expression on a bacterial lawn. The focus of our investigation was on a simple two-component Reaction-Diffusion system, allowing for simple spatial 'patterning' of gene expression. The simplest of these patterns mimic the spots and stripes seen on animal coats. In 1952, Alan Turing famously described this Reaction-Diffusion system and suggested it as the basis for self-organization and pattern formation in biological systems. <br /><b> This is a first step in the direction of engineering multicellular behaviour. </b> <a href="https://2008.igem.org/Team:Cambridge/Signalling"> Read on...</a>
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          <h1>Bacillus</h1>
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To build more complex cellular systems, new tools and techniques are required. We are generating standardized parts, tools, and techniques for the gram-positive chassis ''B. subtillis''. Easy to handle and transform, this bacterium offers many adantages to ''E. coli', including the ability to secrete proteins and integrate DNA into the chromosome. We have designed, built, and submitted gram-positive RBSes, promoters, and shuttle vectors.<br /> <b> As a part of this work we have confirmed single copy chromosomal insertion, demonstrated InFusion assembly, and characterized an improved GFP variant. </b>
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<a href="https://2008.igem.org/Team:Cambridge/Bacillus"> Read on...</a>
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Once all the data is here, we will begin formatting everything properly with CSS and HTML. Here are temporary links to the subsections:
 
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*[[Team:Cambridge/Voltage]] -> Chris - Done
 
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*[[Team:Cambridge/Voltage/Experiments]] -> Chris - Not moving
 
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*[[Team:Cambridge/Voltage/Lab Work]] <-  Done up to and including Sept 2nd. CH EDIT: Done up to 26th Sept - JW
 
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Kath (28/10 16:55)
 
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* I'm saving the html scripts I've changed in case anyone needs them back! Templates 08 and 08a Done. Protocols page done.
 
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* Please note UK spelling! So double L for signalling and Modelling pages please! Please ammend your own links if you've got it wrong.
 
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* Noticed that the two titles by the end of the B.subtilis transformation protocol page have no content. What's that? Perhaps an answer from Ian? Dan? Marie?
 
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          <h1>Modelling</h1>
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We have introduced a model of the AGR quorum-sensing system of S.aureus to illustrate how a typical quorum-sensing system works. The model predicts theoretical values for biological parameters (such as the threshold cell density) that can be verified and we will be also be able to predict how the system behaves if we change a number crucial parameters. This can be extremely useful in informing design decisions when building a synthetic device. We have also expanded this model into a hypothetical setup with a second parallel agr-system. <br /><b> Using this parallel signals model, we investigate how to engineer a biological patterning system.</b> <a href="https://2008.igem.org/Team:Cambridge/Modelling">Read on...</a>
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     <td width="200"><a href="http://www.labtech.co.uk/" class="noborder"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/labtech.jpg" alt="labtech" width="131" height="17"></a></td>
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     <td width="200"><a href="http://www.clontech-europe.com/"><img src="http://www.gen.cam.ac.uk/Images/logos/iGEMsponsors/clontech.jpg" alt="Clontech" width="112" height="34"></a></td>
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Latest revision as of 02:53, 30 October 2008

Home   Voltage   Signalling   Bacillus   Modelling   Protocols   People  
Signalling Bacillus Voltage Modelling

Overview

Since the emergence of Synthetic Biology, bacteria have been engineered to perform a wide variety of simple tasks. They can be made to express proteins, respond to their environment and communicate primitively with each other. Presently, a key goal for the field is to create a communicating, organised and differentiated population of bacteria that can be considered a multicellular organism, capable of performing even more complex tasks. The ultimate goal for this line of research would be to mimic a brain, the most complex structure in the universe. To realize this goal requires the development of systems for rapid, robust communication and self-organised differentiation.
Our project sets the foundation for future research in engineered multi-cellularity by pursuing electrical and peptide signalling, and cellular self-differentiation through spontaneous spatial patterning.

Voltage

In order to simulate neural activity in bacteria, a mechanism resembling a synapse is necessary. At the synapse, neurotransmitter molecules are released from the presynaptic plasma membrane. The neurotransmitter diffuses through the synaptic cleft and binds to chemical receptor molecules on the membrane of the postsynaptic cell. These receptors cause ion channels to open so that ions rush out, changing the transmembrane potential. Attempting to mimic this in a prokaryotic system is particularly attractive as, in a more general sense, it provides an interface between chemical or biological and electrical systems.
Using the amino acid glutamate as our 'neurotransmitter', we have successfully demonstrated a voltage response in bacterial cells. Read on...

Signalling

Using peptide-based signalling systems from gram-positive bacteria, we have laid the foundations for a self-organising biological system, capable of expressing spatial patterns of GFP expression on a bacterial lawn. The focus of our investigation was on a simple two-component Reaction-Diffusion system, allowing for simple spatial 'patterning' of gene expression. The simplest of these patterns mimic the spots and stripes seen on animal coats. In 1952, Alan Turing famously described this Reaction-Diffusion system and suggested it as the basis for self-organization and pattern formation in biological systems.
This is a first step in the direction of engineering multicellular behaviour. Read on...

Bacillus

To build more complex cellular systems, new tools and techniques are required. We are generating standardized parts, tools, and techniques for the gram-positive chassis ''B. subtillis''. Easy to handle and transform, this bacterium offers many adantages to ''E. coli', including the ability to secrete proteins and integrate DNA into the chromosome. We have designed, built, and submitted gram-positive RBSes, promoters, and shuttle vectors.
As a part of this work we have confirmed single copy chromosomal insertion, demonstrated InFusion assembly, and characterized an improved GFP variant. Read on...

Modelling

We have introduced a model of the AGR quorum-sensing system of S.aureus to illustrate how a typical quorum-sensing system works. The model predicts theoretical values for biological parameters (such as the threshold cell density) that can be verified and we will be also be able to predict how the system behaves if we change a number crucial parameters. This can be extremely useful in informing design decisions when building a synthetic device. We have also expanded this model into a hypothetical setup with a second parallel agr-system.
Using this parallel signals model, we investigate how to engineer a biological patterning system. Read on...
labtech Clontech expressys
invitrogen geneservice cambridge bioscience
zymo research VWR Microzone
Finnzymes Fisher Scientific DNA 2.0

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