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(New page: {{Imperial/StartPage2}} IPTG is an inducer which binds to LacI. LacI is a repressor that is constitutively expressed. It binds to the promoter preventing the promoter from expressing an...)
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{{Imperial/StartPage2}}
 
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IPTG is an inducer which binds to LacI.
 
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LacI is a repressor that is constitutively expressed.  It binds to
 
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the promoter preventing the promoter from expressing an output, in
 
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this case GFP.
 
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In the absence of IPTG , LacI is constitutively expressed.  Hence
 
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the constitutive expression model from the previous
 
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characterisation step can be assumed.  At the same time, it can
 
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bind to the promoter to repress it.
 
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[[Image:Repression.JPG|600px]]
 
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[[Image:Equations_plac.JPG‎|600px]]
 
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'''Evolution of LacI concentration'''
 
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[[Image:Conc_LacI.jpg|300px]]
 
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The repressor only binds to the promoter when it is in its unbound
 
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form.
 
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'''Evolution of promoter concentration'''
 
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[[Image:Free_promoters.jpg|300px]]
 
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'''Evolution of the promoter-LacI complex'''
 
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[[Image:Conc_PLacI_Complex.jpg|300px]]
 
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'''Evolution of GFP'''
 
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[[Image:conc_FP.jpg|300px]]
 
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d<sub>GFP</sub>[GFP] is the basal rate of GFP. This is caused by
 
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the leakiness of the LacI-PLac complex when LacI does not repress
 
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the promoter completely.
 
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{{Imperial/EndPage|Growth_Curve|Motility}}
 

Latest revision as of 03:03, 30 October 2008