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| - | {{Imperial/StartPage2}}
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| - | IPTG is an inducer which binds to LacI.
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| - | LacI is a repressor that is constitutively expressed. It binds to
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| - | the promoter preventing the promoter from expressing an output, in
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| - | this case GFP.
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| - | In the absence of IPTG , LacI is constitutively expressed. Hence
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| - | the constitutive expression model from the previous
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| - | characterisation step can be assumed. At the same time, it can
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| - | bind to the promoter to repress it.
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| - | [[Image:Repression.JPG|600px]]
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| - | [[Image:Equations_plac.JPG|600px]]
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| - | '''Evolution of LacI concentration'''
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| - | [[Image:Conc_LacI.jpg|300px]]
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| - | The repressor only binds to the promoter when it is in its unbound
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| - | form.
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| - | '''Evolution of promoter concentration'''
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| - | [[Image:Free_promoters.jpg|300px]]
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| - | '''Evolution of the promoter-LacI complex'''
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| - | [[Image:Conc_PLacI_Complex.jpg|300px]]
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| - | '''Evolution of GFP'''
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| - | [[Image:conc_FP.jpg|300px]]
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| - | d<sub>GFP</sub>[GFP] is the basal rate of GFP. This is caused by
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| - | the leakiness of the LacI-PLac complex when LacI does not repress
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| - | the promoter completely.
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| - | {{Imperial/EndPage|Growth_Curve|Motility}}
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Latest revision as of 03:03, 30 October 2008
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